專長與興趣：

Plant molecular biology and cell biology.

1.    Regulation of chloroplast development

Chloroplast is an important organelle in plants due to its unique function in providing oxygen and most chemical energy on earth by photosynthesis. Even though chloroplasts contain their own DNA and are capable to express the genomic information in their DNA, most of the chloroplast proteins are encoded by the nuclear genome and then transported into chloroplasts. Our research focus is using molecular and biochemical techniques to study the functions of CIA2 gene in Arabidopsis. CIA2 is a transcription regulator which controls the expression of genes encoding chloroplast translocons, ribosomal proteins, and chaperonin. In consequence, CIA2 regulates the chloroplast development by coordinating the efficiency of chloroplast protein import and protein synthesis.

2.    Regulatory mechanism of ubiquitin gene expression

Ubiquitin, a highly conserved protein in eukaryotic organisms, is generally participated in ATP-dependent proteolysis, DNA repair, cell cycle control, maintenance of chromatin structure, stress response, cell-cell interaction, signal transduction, endocytosis, viral infection, and regulation of plant physiological responses. In Arabidopsis, ubiquitin is encoded by five polyubiquitin and four ubiquitin extension genes. We use biochemical, genetic, and bioinformatic approaches to reveal the regulatory mechanism of differential expression of these ubiquitin genes.

A. 期刊論文    

1. Sun CW*, Huang YC, and Chang HY (2009). CIA2 coordinately up-regulates protein import and synthesis in leaf chloroplasts. Plant Physiology, 150: 879-888. (SCI, IF=6.367) 
2. Lin CS, Liu NT, Liao DC, Yu JS, Tsao CH, Lin CH, Sun CW, Jane WN, Tsay HS, Chen JJW, Lai EM, Lin NS, Chang WC, Lin CC (2008). Differential protein expression of two photosystem II subunits, PsbO and PsbP in an albino mutant of Bambusa edulis with chloroplast DNA aberration. J. American Society of Horticultural Science, 133: 1–8. (SCI, IF=0.915)
3. Huang LC, Lai UL, Yang SF, ChuMJ, Kuo CI, Tsai MF and Sun CW* (2007). Delayed flower senescence of Petunia hybrida plants transformed with antisense broccoli ACC synthase and ACC oxidase genes. Postharvest Biology and Technology, 46: 47–53. (SCI, IF=1.59)
4. Chang SC and Sun CW* (2007). Effects of environmental factors on expression of ubiquitin gene family in rice. BioFormosa, 42: 35-45.  
5. Wu TH and Sun CW* (2007). Construction and analysis of EST library in Wolfiporia cocos. BioFormosa 42: 47-53.  
6. Lin CS, Lai YH, Sun CW, Liu NT, Tsay HS, Chang WC and Chen JW (2006). Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis. Plant Cell Tissue and Organ Culture, 86:169–175. (SCI, IF=1.068)
7. Hung WF, Chen LJ, Boldt R, Sun CW and Li HM (2004). Characterization of Arabidopsis glutamine phosphoribosyl pyrophosphate amidotransferase-deficient mutants. Plant Physiology, 135: 1314-1323. (SCI, IF=6.367)
8. Sun CW, Chen LJ, Lin LC and Li HM (2001). Leaf-specific up-regulation of chloroplast translocon genes by a CCT motif-containing protein, CIA2. The Plant Cell, 13: 2053-2061. (SCI, IF=9.653)
9. Chen LF, Hwang JY, Charng YY, Sun CW and Yang SF (2001). Transformation of broccoli (Brassica oleracea var. italica) with isopenyltransferase gene via Agrobacterium tumefaciens for post-harvest yellowing retardation. Molecular Breeding, 7: 243-257. (SCI, IF=2.357)
10. Sun CW and Callis J (1997). Independent modulation of Arabiodopsis thaliana polyubiquitin genes mRNA levels under different developmental and environmental conditions. The Plant Journal, 11: 1017-1027. (SCI, IF=6.751)
11. Sun CW, Griffen S and Callis J (1997). Structure differences of polyubiquitin genes in different Arabidopsis ecotypes. Plant Molecular Biology, 34: 745-758. (SCI, IF=3.847)
12. Callis J, Carpenter T, Sun CW and Vierstra R (1995). Structure and evolution of genes encoding polyubiquitin and ubiquitin-like proteins in Arabidopsis thaliana ecotype Columbia. Genetics, 139: 921-939. (SCI, IF=4.001)
13. Sun CW and Callis J (1993). Recent stable insertion of mitochondrial DNA into an Arabidopsis polyubiquitin gene by nonhomologous recombination. The Plant Cell, 5: 97-107. (SCI, IF=9.653) 

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C. 研究計畫 

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